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1.
Eur J Immunol ; 40(7): 2035-40, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20411563

RESUMO

The receptor for IgA, FcalphaRI or CD89, is expressed on myeloid cells and can trigger phagocytosis, tumor cell lysis, and release of inflammatory mediators. These functions critically depend on the associated FcR gamma-chain; however, some biological functions, like receptor internalization, are solely mediated by FcalphaRI alpha-chain. Little is known as to how FcalphaRI regulates these processes and the FcalphaRI intracellular domain does not contain recognized signalling motifs. We searched for associating proteins and identified c-Jun activating binding protein 1 (JAB1) as a binding partner specifically for FcalphaRI. We found increased FcalphaRI surface expression after ectopic expression of JAB1 as well as diminished protein levels of total FcR gamma-chain levels after JAB1 knock-down. These data functionally link JAB1 with controlling protein expression levels of FcalphaRI-FcR gamma-chain protein complex.


Assuntos
Antígenos CD/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Peptídeo Hidrolases/metabolismo , Receptores Fc/metabolismo , Receptores de IgG/biossíntese , Motivos de Aminoácidos/genética , Animais , Antígenos CD/genética , Complexo do Signalossomo COP9 , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/genética , Peptídeos e Proteínas de Sinalização Intracelular/imunologia , Camundongos , Células NIH 3T3 , Peptídeo Hidrolases/genética , Peptídeo Hidrolases/imunologia , Ligação Proteica/genética , Domínios e Motivos de Interação entre Proteínas/genética , Estabilidade Proteica , RNA Interferente Pequeno/genética , Receptores Fc/genética , Receptores de IgG/genética , Ativação Transcricional/genética , Transgenes/genética , Células U937
2.
J Immunol ; 176(6): 3603-10, 2006 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-16517729

RESUMO

The prototypic receptor for IgA (FcalphaRI, CD89) is expressed on myeloid cells and can trigger phagocytosis, tumor cell lysis, and release of inflammatory mediators. The functions of FcalphaRI and activating receptors for IgG (FcgammaRI and FcgammaRIII) are dependent on the FcR gamma-chain dimer. This study increases our understanding of the molecular basis of the FcalphaRI-FcR gamma-chain transmembrane interaction, which is distinct from that of other activatory FcRs. FcalphaRI is unique in its interaction with the common FcR gamma-chain, because it is based on a positively charged residue at position 209, which associates with a negatively charged amino acid of FcR gamma-chain. We explored the importance of the position of this positive charge within human FcalphaRI for FcR gamma-chain association and FcalphaRI functioning with the use of site-directed mutagenesis. In an FcalphaRI R209L/A213H mutant, which represents a vertical relocation of the positive charge, proximal and distal FcR gamma-chain-dependent functions, such as calcium flux, MAPK phosphorylation, and IL-2 release, were similar to those of wild-type FcalphaRI. A lateral transfer of the positive charge, however, completely abrogated FcR gamma-chain-dependent functions in an FcalphaRI R209L/M210R mutant. By coimmunoprecipitation, we have demonstrated the loss of a physical interaction between FcR gamma-chain and FcalphaRI M210R mutant, thus explaining the loss of FcR gamma-chain-dependent functions. In conclusion, not only the presence of a basic residue in the transmembrane region of FcalphaRI, but also the orientation of FcalphaRI toward the FcR gamma-chain dimer is essential for FcR gamma-chain association. This suggests the involvement of additional amino acids in the FcalphaRI-FcR gamma-chain interaction.


Assuntos
Antígenos CD/genética , Antígenos CD/metabolismo , Mutação/genética , Receptores Fc/genética , Receptores Fc/metabolismo , Receptores de IgG/imunologia , Receptores de IgG/metabolismo , Transdução de Sinais , Sequência de Aminoácidos , Animais , Antígenos CD/imunologia , Cálcio/metabolismo , Linhagem Celular , Membrana Celular/metabolismo , Humanos , Interleucina-2/biossíntese , Ligantes , Camundongos , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Dados de Sequência Molecular , Fosforilação , Ligação Proteica , Receptores Fc/imunologia , Receptores de IgG/genética , Alinhamento de Sequência
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